Reggie-1 and reggie-2, two cell surface proteins expressed by retinal ganglion cells during axon regeneration

• DOI: 10.1242/dev.124.2.577
• Publication Date: 2021-04-26T03:06:26Z
• Authors: Thomas Schulte; Katja A. Paschke; Ute Laessing; Friedrich Lottspeich; Claudia A. O. Stu...
• Keywords: Retinal regeneration

ABSTRACT Fish – in contrast to mammals regenerate retinal ganglion cell axons when the optic nerve is severed. Optic injury leads reexpression of proteins, which typically are first expressed newly differentiated cells and axons. Here we identified two new proteins fish cells, reggie-1 reggie-2, with monoclonal antibody M802 molecular cloning techniques. In normal fish, stained few derived from newborn added lifelong margin. After injury, however, labeled all throughout their path into tectum. Consistent staining, reggie-2 mRNAs were present lesioned as demonstrated by situ hybridization, but not detectable mature counterparts. western blots membrane adult goldfish brain, recognizes a 48×103Mr protein band. At amino acid level, 44% identical, lack transmem-brane anchor domains, appear associated ionic interactions. Reggie-1 homologous 35×103Mr ESA (human epidermal surface antigen) here neuronal on at margin reex-pressed upon during axonal regeneration.