An entire functional mammary gland may comprise the progeny from a single cell

• DOI: 10.1242/dev.125.10.1921
• Publication Date: 2021-04-26T03:13:28Z
• Authors: Edith C. Kordon; Gilbert H. Smith
• Keywords: 0301 basic medicine; Stem Cells; Epithelial Cells; 3. Good health; Mice; 03 medical and health sciences; Mammary Glands, Animal; Mammary Tumor Virus, Mouse; Proviruses; DNA, Viral; Animals; Female; Cell Division

Any epithelial portion of a normal mouse mammary gland can reproduce an entire functional when transplanted into epithelium-free fat pad. Mouse hyperplasias and tumors are clonal dominant populations probably represent the progeny single transformed cell. Our study provides evidence that multipotent stem cells positioned throughout mature fully developed have capacity to produce sufficient differentiated recapitulate gland. also demonstrates these self-renewing found with undiminished capacities in newly regenerated We taken advantage experimental model where tumor virus infects inserts deoxyribonucleic acid copy(ies) its genome during replication. The insertions occur randomly within somatic genome. CzechII mice no endogenous nucleic sequence homology virus; therefore all viral may be detected by Southern analysis provided number contain specific insertional event. Transplantation random fragments infected produced clonal-dominant pads. Serial transplantation pieces clonally derived outgrowths second generation glands possessing same insertion sites providing for self-renewal original Limiting dilution studies cell cultures from third demonstrated three but distinct progenitors were present populations. Estimation potential evolved individual mammary-specific is order 10(12)-10(13). Therefore, one might easily account renewal epithelium over several transplant generations.