Mouse primordial germ cells lacking β1 integrins enter the germline but fail to migrate normally to the gonads

• DOI: 10.1242/dev.126.8.1655
• Publication Date: 2021-04-26T03:59:15Z
• Authors: Robert Anderson; Reinhard Fässler; Elisabeth Georges...; Richard O. Hynes; Bernhard L. Bader; Jordan A. Kreidberg; Kyle Schaible; Janet Heasman; Christopher Wylie
• Keywords: Germ line development

Abstract Primordial germ cells are the founder of gametes. They set aside at initial stages gastrulation in mammals, become embedded hind-gut endoderm, then actively migrate to sites gonad formation. The molecular basis this migration is poorly understood. Here we sought determine if members integrin family cell surface receptors required for primordial migration, as integrins have been implicated several other motile types. We established a line mice which express green fluorescent protein germline that has enabled us efficiently purify different by flow cytometry. catalogued spectrum subunit expression during and after using cytometry, immunocytochemistry RT-PCR. Through analysis β1−/−→wild-type chimeras, show embryonic lacking β1 can enter germline. However, β1−/− do not colonize efficiently. Embryos with targeted deletion α3, α6, or αV no major defects migration. These results demonstrate role β1-containing development germline, although an equivalent α subunit(s) yet be established.