An isoform of eIF4E is a component of germ granules and is required for spermatogenesis inC. elegans
Male
0301 basic medicine
DNA, Complementary
Base Sequence
Disorders of Sex Development
Temperature
Gene Expression Regulation, Developmental
RNA-Binding Proteins
Cytoplasmic Granules
03 medical and health sciences
Eukaryotic Initiation Factor-4E
Peptide Initiation Factors
Infertility
Protein Biosynthesis
Animals
Protein Isoforms
Female
RNA, Messenger
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Spermatogenesis
DOI:
10.1242/dev.128.20.3899
Publication Date:
2021-04-26T04:36:27Z
AUTHORS (7)
ABSTRACT
Control of gene expression at the translational level is crucial for many developmental processes. The mRNA cap-binding protein, eIF4E, is a key player in regulation of translation initiation; appropriate levels of eIF4E are essential for normal cell-cycle regulation and tissue differentiation. The observation that eIF4E levels are elevated during gametogenesis in several organisms suggests that eIF4E might have a specific role in gamete formation as well. We show that one of the five isoforms of C. elegans eIF4E, IFE-1, is enriched in the germline and is a component of germ granules (P granules). The association of IFE-1 with P granules requires the P-granule protein PGL-1. In vitro PGL-1 interacts directly with IFE-1, but not with the other four isoforms of eIF4E. Analysis of animals depleted of IFE-1 by RNAi shows that IFE-1 is required for spermatogenesis, specifically for efficient progression through the meiotic divisions and for the production of functional sperm, in both hermaphrodites and males. The requirement for IFE-1 is highly sensitive to temperature. IFE-1 is not required for oogenesis, as ife-1(RNAi) hermaphrodites produce viable progeny when normal sperm are supplied. Consistent with a primary role in spermatogenesis, ife-1 mRNA levels are highest in regions of the gonad undergoing spermatogenesis. Our results suggest that C. elegans spermatogenesis requires either this specific isoform of eIF4E or an elevated level of eIF4E.
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