Control ofDrosophilaimaginal disc development byrotundandroughened eye: differentially expressed transcripts of the same gene encoding functionally distinct zinc finger proteins

• DOI: 10.1242/dev.129.5.1273
• Publication Date: 2021-04-25T14:25:05Z
• Authors: Susan E. St Pierre; Maximo I. Galindo; Juan P. Couso; Stefan Thor
• Keywords: Eye Proteins

The Drosophila rotund gene is required in the wings, antenna, haltere, proboscis and legs. A member of Rac family GTPases, denoted racGAP gene, was previously identified region. However, previous studies indicated that not responsible for phenotypes had yet to be identified. We have isolated show it a Krüppel zinc finger genes. adjacent roughened eye locus specifically affects genetically separable from rotund. are tightly linked, we therefore also transcript. Intriguingly, part but represented by different transcripts result utilization two promoters direct expression non-overlapping domains larval imaginal discs. predicted Rotund Roughened Eye proteins share same C-terminal region, including domain, differ their N-terminal regions. Each cDNA can rescue only corresponding mutation negative effects when expressed each others domain expression. These results indicate addition differential eye, distinct activities. act downstream early patterning genes such as dachshund appear involved Notch signaling regulating Delta, scabrous Serrate.