BDNF stimulates migration of cerebellar granule cells
Mice, Knockout
0301 basic medicine
Mice
03 medical and health sciences
Microscopy, Video
Cell Movement
Brain-Derived Neurotrophic Factor
Cerebellum
Animals
Gene Expression Regulation, Developmental
In Vitro Techniques
DOI:
10.1242/dev.129.6.1435
Publication Date:
2021-04-23T20:24:13Z
AUTHORS (9)
ABSTRACT
During development of the nervous system, neural progenitors arise in proliferative zones, then exit the cell cycle and migrate away from these zones. Here we show that migration of cerebellar granule cells out of their proliferative zone, the external granule cell layer (EGL), is impaired in Bdnf–/– mice. The reason for impaired migration is that BDNF directly and acutely stimulates granule cell migration. Purified Bdnf–/– granule cells show defects in initiation of migration along glial fibers and in Boyden chamber assays. This phenotype can be rescued by exogenous BDNF. Using time-lapse video microscopy we find that BDNF is acutely motogenic as it stimulates migration of individual granule cells immediately after addition. The stimulation of migration reflects both a chemokinetic and chemotactic effect of BDNF. Collectively, these data demonstrate that BDNF is directly motogenic for granule cells and provides a directional cue promoting migration from the EGL to the internal granule cell layer (IGL).Movies available on-line
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