Lmx1a functions in intestinal serotonin-producing enterochromaffin cells downstream of Nkx2.2

• DOI: 10.1242/dev.130682
• Publication Date: 2016-06-11T04:31:44Z
• Authors: Stefanie Gross; Diana C. Garofalo; Dina A. Balderes; Teresa L. Mastracci; José M. Dias; Thomas Perlmann; Johan Ericson; Lori Sussel
• Keywords: Enterochromaffin cell; Enteroendocrine cell

Intestinal hormone-producing cells represent the largest endocrine system in body, but remarkably little is known about enteroendocrine cell type specification embryo and adult. We analyzed stage- type-specific deletions of Nkx2.2 its functional domains order to characterize role development maintenance lineages mouse duodenum colon. Although regulates at all stages examined, it controls differentiation progressively fewer populations when deleted from Ngn3(+) progenitor or adult duodenum. During embryonic types, except gastrin preproglucagon. In developing cells, not required for neuropeptide Y vasoactive intestinal polypeptide, indicating that a subset these derive an Nkx2.2-independent lineage. duodenum, becomes dispensable cholecystokinin secretin production. mutant conditions, serotonin-producing enterochromaffin were most severely reduced lineage determined transcription factor Lmx1a expressed functions downstream Nkx2.2. Lmx1a-deficient mice have expression Tph1, rate-limiting enzyme serotonin biosynthesis. These data clarify function homeostatic populations, identify as novel marker also essential production biosynthetic Tph1.