BAF chromatin remodelling complex is an epigenetic regulator of lineage specification in the early mouse embryo

• DOI: 10.1242/dev.131961
• Publication Date: 2016-03-08T02:40:01Z
• Authors: Maryna Panamarova; Andy Cox; Krzysztof Wicher; Richard Butler; Natalia Bulgakova; Shin Jeon; Barry Rosen; Rho H. Seong; William Skarnes; Gerald Crabtree; Magdalena Zernick...
• Keywords: Ectopic expression

Dynamic control of gene expression is essential for development a totipotent zygote into an embryo with defined cell lineages. The accessibility genes responsible specification to transcriptional machinery dependent on chromatin remodelling complexes such as the SWI\SNF (BAF) complex. However, role BAF complex in early mouse has remained unclear. Here we demonstrate that BAF155, major subunit, regulates assembly vivo, and lineage blastocyst. We find associations BAF155 other subunits become enriched extra-embryonic lineages just prior implantation. This enrichment attributed decreased mobility compared embryonic lineage. Down-regulation leads increased pluripotency marker Nanog its ectopic lineages, whereas up-regulation differentiation markers. Finally, show arginine methyltransferase CARM1 methylates which influences between Together our results indicate novel BAF-dependent via regulation specification.