Non-cell autonomous control of precerebellar neuron migration by Slit and Robo proteins

• DOI: 10.1242/dev.150375
• Publication Date: 2017-12-20T01:20:25Z
• Authors: Chloé Dominici; Quentin Rappeneau; Pavol Zelina; Stéphane Fouquet; Alain Chédotal
• Keywords: Slit

During development, precerebellar neurons migrate tangentially from the dorsal hindbrain to floor plate. Their axons cross it but their cell bodies stop ventral migration upon reaching midline. It has previously been shown that Slit chemorepellents and receptors, Robo1 Robo2, might control of in a repulsive manner. Here, we have used conditional knockout strategy mice test this hypothesis. We show targeted inactivation expression Robo2 receptors does not perturb they still at The selective ablation all three proteins floor-plate cells no effect on pontine only induces small subset inferior olivary across Likewise, facial nucleus is dispensable for neuron migration. Together, these results act non-cell autonomously migrating signals, other than proteins, must exist prevent midline crossing.