Regulation of the mammalian maternal-to-embryonic transition by eukaryotic translation initiation factor 4E

• DOI: 10.1242/dev.190793
• Publication Date: 2021-05-20T08:24:02Z
• Authors: Yan Li; Jianan Tang; Xu Ji; Min-Min Hua; Miao Liu; Lu Chang; Yihua Gu; Changgen Shi; Wuhua Ni; Jing Liu; Hui-juan Shi; Xuefeng Huang; Christopher O'Neill; Xingliang Jin
• Keywords: EIF4E

ABSTRACT Eukaryotic translation initiation factor 4E (eIF4E) mediates cap-dependent translation. Genetic and inhibitor studies show that eIF4E expression is required for the successful transition from maternal to embryonic control of mouse embryo development. was present in oocyte cytoplasm soon after fertilization during each stage early Functional knockout (Eif4e−/−) by PiggyBac [Act-RFP] transposition resulted peri-implantation lethality because failure normal epiblast formation. Maternal stores supported development up two- four-cell stage, which new occurred both paternal inherited alleles. Inhibition maternally acquired (using 4EGI-1) a block at two-cell stage. activity protein synthesis Eif4e−/− embryos had lower translational compared with wild-type embryos. eIF4E-binding 1 (4E-BP1) hypophosphorylation-dependent negative regulator eIF4E. mTOR 4E-BP1 phosphorylation inhibiting retarded Thus, this study shows regulated key transitions mammalian essential