DamID transcriptional profiling identifies the Snail/Scratch transcription factor Kahuli as an Alk target in the Drosophila visceral mesoderm

• DOI: 10.1242/dev.199465
• Publication Date: 2021-12-14T16:58:40Z
• Authors: Patricia Mendoza-...; Swaraj Basu; Sanjay Kumar Sukumar; Badrul Arefin; Georg Wolfstetter; Vimala Anthonydhason; Linnea Molander; Ezgi Uçkun; Henrik Lindehell; Cristina Lebrero-...; Jan Larsson; Erik Larsson; Mats Bemark; Ruth H. Palmer
• Keywords: 0301 basic medicine; Midgut constriction; Cell- och molekylärbiologi; ChIP; Embryonic Development; Nerve Tissue Proteins; TaDa; Muscle Development; Biochemistry; Pointed; Mesoderm; 03 medical and health sciences; Proto-Oncogene Proteins; Animals; Drosophila Proteins; Single cell; Anaplastic Lymphoma Kinase; RNA, Messenger; RNA-Seq; Biokemi; Molecular Biology; Jelly belly; 0303 health sciences; Molekylärbiologi; Gene Expression Profiling; Muscles; Gene Expression Regulation, Developmental; Genetics and Genomics; Genetik och genomik; Cell Differentiation; Signaling; DNA-Binding Proteins; Drosophila melanogaster; Single-Cell Analysis; Cell and Molecular Biology; ETS; Research Article; Signal Transduction; Transcription Factors

ABSTRACT Development of the Drosophila visceral muscle depends on Anaplastic Lymphoma Kinase (Alk) receptor tyrosine kinase (RTK) signaling, which specifies founder cells (FCs) in circular mesoderm (VM). Although Alk activation by its ligand Jelly Belly (Jeb) is well characterized, few target molecules have been identified. Here, we used targeted DamID (TaDa) to identify targets embryos overexpressing Jeb versus with abrogated activity, revealing differentially expressed genes, including Snail/Scratch family transcription factor Kahuli (Kah). We confirmed Kah mRNA and protein expression VM, identified midgut constriction defects mutants similar those pointed (pnt). ChIP RNA-Seq data analysis defined a target-binding site that Snail, set common genes putatively regulated Pnt during constriction. Taken together, report rich dataset Alk-responsive loci embryonic VM functionally characterize role regulation morphogenesis.