A single-cell atlas of mouse lung development

• DOI: 10.1242/dev.199512
• Publication Date: 2021-12-20T11:22:42Z
• Authors: Nicholas M. Negretti; Erin J. Plosa; John T. Benjamin; Bryce A. Schuler; A. Christian Habe...; Christopher S. Je...; Peter Gulleman; Claire Bunn; Alice N. Hackett; Meaghan Ransom; Chase J. Taylor; David Nichols; Brittany K. Matlock; Susan H. Guttentag; Timothy S. Blackwell; Nicholas E. Banovich; Jonathan A. Kropski; Jennifer M. S. Sucre
• Keywords: Organogenesis; Parenchyma

ABSTRACT Lung organogenesis requires precise timing and coordination to effect spatial organization function of the parenchymal cells. To provide a systematic broad-based view mechanisms governing dynamic alterations in cells over crucial periods development, we performed single-cell RNA-sequencing time-series yielding 102,571 epithelial, endothelial mesenchymal across nine time points from embryonic day 12 postnatal 14 mice. Combining computational fate-likelihood prediction with RNA situ hybridization immunofluorescence, explore lineage relationships during saccular alveolar stage transition. The utility this publicly searchable atlas resource (www.sucrelab.org/lungcells) is exemplified by discoveries complexity type 1 pneumocyte characterization Wnt expression patterns stages – wherein major expansion gas-exchange surface occurs. We an integrated cellular dynamics cell populations lung organogenesis.