Laser-mediated osteoblast ablation triggers a pro-osteogenic inflammatory response regulated by reactive oxygen species and glucocorticoid signaling in zebrafish

• DOI: 10.1242/dev.199803
• Publication Date: 2022-04-29T08:43:02Z
• Authors: Karina Geurtzen; Alejandra Cristin...; Ankita Duseja; Anastasia Kurzyukova; Franziska Knopf
• Keywords: 0301 basic medicine; Macrophage; MIGRATION; IMMUNE; Lineage tracing; 03 medical and health sciences; Glucocorticoid; HYDROGEN-PEROXIDE; REGENERATION; Animals; OXIDATIVE STRESS; MACROPHAGES; Glucocorticoids; NEUTROPHILS; Zebrafish; Science & Technology; Osteoblasts; SPINAL MOTOR-NEURONS; Osteoblast; Lasers; FIN; 3. Good health; Reactive oxygen species; BONE-CELLS; Reactive Oxygen Species; Life Sciences & Biomedicine; Developmental Biology

ABSTRACT In zebrafish, transgenic labeling approaches, robust regenerative responses and excellent in vivo imaging conditions enable precise characterization of immune cell behavior response to injury. Here, we monitored osteoblast-immune interactions bone, a tissue which is particularly difficult image tetrapod species. Ablation individual osteoblasts leads recruitment neutrophils macrophages varying numbers, depending on the extent initial insult, initiates generation cathepsin K+ osteoclasts from macrophages. Osteoblast ablation triggers production pro-inflammatory cytokines reactive oxygen species, are needed for successful macrophage recruitment. Excess glucocorticoid signaling as it occurs during stress inhibits recruitment, maximum speed changes phenotype. Although osteoblast loss compensated within day by contribution committed osteoblasts, continue populate region. Their presence required fill lesion site. Our model enables visualization bone repair after microlesions at single-cell resolution demonstrates pro-osteogenic function tissue-resident non-mammalian vertebrates.