Derivation of extra-embryonic and intra-embryonic macrophage lineages from human pluripotent stem cells

• DOI: 10.1242/dev.200016
• Publication Date: 2022-02-18T09:26:10Z
• Authors: Andrea L. Bredemeyer; Junedh M. Amrute; Andrew L. Koenig; Rachel A. Idol; Li He; Stephanie A. Luff; Carissa Dege; Jamison M. Leid; Joel D. Schilling; J. Travis Hinson; Mary C. Dinauer; Christopher M. St...; Kory J. Lavine
• Keywords: Regenerative Medicine

Tissue-resident macrophages are increasingly recognized as important determinants of organ homeostasis, tissue repair, remodeling and regeneration. Although the ontogeny function tissue-resident has been identified distinct from postnatal hematopoiesis, inability to specify, in vitro, similar populations that recapitulate these developmental waves limited our ability study their potential for regenerative applications. We took advantage concept monocyte-derived originate extra-embryonic definitive hematopoietic lineages devise a system generate pure cultures resemble or subsets. demonstrate human pluripotent stem cell-derived extra-embryonic-like intra-embryonic-like progenitors differentiate into morphologically, transcriptionally functionally macrophage populations. Single-cell RNA sequencing developing mature uncovered trajectories gene expression programs derived progenitors. These findings establish resource generation resident-like specification under defined conditions explore use engineering medicine