High-resolution dynamic mapping of the C. elegans intestinal brush border

• DOI: 10.1242/dev.200029
• Publication Date: 2021-10-27T10:10:01Z
• Authors: Aurélien Bidaud-M...; Flora Demouchy; Ophélie Nicolle; Anne Pacquelet; Shashi Kumar Suman; Camille N. Plancke; François B. Robin; Grégoire Michaux
• Keywords: Brush border

ABSTRACT The intestinal brush border is made of an array microvilli that increases the membrane surface area for nutrient processing, absorption and host defense. Studies on mammalian cultured epithelial cells have uncovered some molecular players physical constraints required to establish this apical specialized membrane. However, building maintenance a in vivo has not yet been investigated detail. Here, we combined super-resolution imaging, transmission electron microscopy genome editing developing nematode Caenorhabditis elegans build high-resolution dynamic localization map known new markers. Notably, show components are dynamically enriched at during outgrowth maturation, but become highly stable once built. This toolbox will be instrumental understanding processes growth vivo, as well effect genetic perturbations, notably context disorders affecting integrity.