A prominent gene activation role for C-terminal binding protein in mediating PcG/trxG proteins through Hox gene regulation

• DOI: 10.1242/dev.200153
• Publication Date: 2022-06-06T12:16:37Z
• Authors: Cai-Li Bi; Qian Cheng; Ling-Yue Yan; Hong-Yan Wu; Qiang Wang; Ping Wang; Lin Cheng; Rui Wang; Lin Yang; Jian Li; Feng Tie; Hao Xie; Ming Fang
• Keywords: Derepression; Polycomb-group proteins

ABSTRACT The evolutionarily conserved C-terminal binding protein (CtBP) has been well characterized as a transcriptional co-repressor. Herein, we report previously unreported function for CtBP, showing that lowering CtBP dosage genetically suppresses Polycomb group (PcG) loss-of-function phenotypes while enhancing of trithorax (trxG) in Drosophila, suggesting the role gene activation is more pronounced fly development than thought. In cells, show required derepression most direct PcG target genes, which are highly enriched by homeobox transcription factors, including Hox genes. Using ChIP and co-IP assays, demonstrate directly molecular switch between H3K27me3 H3K27ac derepressed loci. addition, physically interacts with many proteins, such UTX, CBP, Fs(1)h RNA Pol II, have roles, potentially assisting their recruitment to promoters response elements control expression. Therefore, reveal prominent confers major epigenetic program segmentation development.