Distinct hyperactive RAS/MAPK alleles converge on common GABAergic interneuron core programs

• DOI: 10.1242/dev.201371
• Publication Date: 2023-05-11T13:24:38Z
• Authors: Sara J. Knowles; April M. Stafford; Tariq Zaman; Kartik Angara; Michael R. Williams; Jason M. Newbern; Daniel Vogt
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; MAP Kinase Signaling System; Interneurons; GABAergic Neurons; Somatostatin; Alleles; 3. Good health; Research Article; Signal Transduction

ABSTRACT RAS/MAPK gene dysfunction underlies various cancers and neurocognitive disorders. Although the roles of RAS/MAPK genes have been well studied in cancer, less is known about their function during neurodevelopment. There are many genes that work in concert to regulate RAS/MAPK signaling, suggesting that if common brain phenotypes could be discovered they could have a broad impact on the many other disorders caused by distinct RAS/MAPK genes. We assessed the cellular and molecular consequences of hyperactivating the RAS/MAPK pathway using two distinct genes in a cell type previously implicated in RAS/MAPK-mediated cognitive changes, cortical GABAergic interneurons. We uncovered some GABAergic core programs that are commonly altered in each of the mutants. Notably, hyperactive RAS/MAPK mutants bias developing cortical interneurons towards those that are somatostatin positive. The increase in somatostatin-positive interneurons could also be prevented by pharmacological inhibition of the core RAS/MAPK signaling pathway. Overall, these findings present new insights into how different RAS/MAPK mutations can converge on GABAergic interneurons, which may be important for other RAS/MAPK genes and related disorders.