Nucleolin functions in nucleolus formation and chromosome congression

• DOI: 10.1242/jcs.008771
• Publication Date: 2007-05-30T01:39:48Z
• Authors: Nan Ma; Sachihiro Matsunaga; Hideaki Takata; Rika Ono-Maniwa; Susumu Uchiyama; Kiichi Fukui
• Keywords: Nucleolin; Prometaphase; Interphase

A complex structure, designated the chromosome periphery, surrounds each during mitosis. Although several proteins have been shown to localize their functions mitosis remain unclear. Here, we used a combination of high-resolution microscopy and RNA-interference-mediated depletion study nucleolin, nucleolar protein localized at in interphase During mitosis, nucleolin was peripheral region including vicinity outer kinetochore chromosomes. Staining with an antibody specific for phosphorylated by CDC2 revealed that also associated spindle poles from prometaphase anaphase. Nucleolin resulted disorganization nucleoli interphase. Furthermore, nucleolin-depleted cells showed prolonged cell cycle misaligned chromosomes defects organization. The syntelic kinetochore-microtubule attachments reduced centromere stretching. Taken together, our results indicate is required nucleolus formation, involved congression formation.