Both mitogen activated protein kinase and the mammalian target of rapamycin modulate the development of functional renal proximal tubules in matrigel

• DOI: 10.1242/jcs.01020
• Publication Date: 2004-03-23T01:13:41Z
• Authors: Ho Jae Han; Wade J. Sigurdson; Peter A. Nickerson; Mary Taub
• Keywords: Lucifer yellow; Matrigel; Tubule; Apical membrane; Wortmannin

Tubules may arise during branching morphogenesis through several mechanisms including wrapping, budding, cavitation and cord hollowing. In this report we present evidence that is consistent with renal proximal tubule formation a process of hollowing (a requires the concomitant establishment apicobasal polarity lumen formation). Pockets filled Lucifer Yellow were observed within developing cords rabbit cells in matrigel. The observation accumulation suggests functional polarization. initially transported intracellularly by means basolaterally oriented p-aminohippurate transport system, followed apical secretion into nephron. Consistent such polarization tubules, Triticum vulgare was to bind lumenal membranes pockets Yellow-filled lumens. As lectin binds apically tubule, T. binding indicative emergence an domain before contiguous lumen. Both epidermal growth factor hepatocyte stimulated transporting tubules. stimulatory effect both on tubulogenesis inhibited PD98059, mitogen activated protein kinase inhibitor, rather than wortmannin, inhibitor phosphoinositide 3-kinase. Nevertheless, lumens tubules formed presence PD98059 as well indicating these drugs did not prevent cavitation. By contrast, rapamycin, mammalian target prevented without affecting frequency cords. Multicellular cysts form 8-bromocyclic AMP-treated cultures. similarly accumulate lumenally, it very likely processes other organic anion are involved cystogenesis, Na,K-ATPase.