p130Cas interacts with estrogen receptor α and modulates non-genomic estrogen signaling in breast cancer cells

• DOI: 10.1242/jcs.01025
• Publication Date: 2004-03-13T00:03:11Z
• Authors: Sara Cabodi; Laura Moro; Germano Baj; Monica Smeriglio; Paola Di Stefano; Silvana Gippone; Nicola Surico; Lorenzo Silengo; Emilia Turco; Guido Tarone; Paola Defilippi
• Keywords: 610; Breast Neoplasms; Retinoblastoma Protein; p130Cas; Estrogen; Estrogen receptor; c-Src; Erk1/2 MAPK; Cyclin D1; Phosphatidylinositol 3-Kinases; 03 medical and health sciences; Cell Line, Tumor; Humans; Cyclin D1; RNA, Small Interfering; Mitogen-Activated Protein Kinase 1; 0303 health sciences; Mitogen-Activated Protein Kinase 3; Retinoblastoma-Like Protein p130; Carcinoma; Estrogen Receptor alpha; Proteins; Estrogens; 3. Good health; Enzyme Activation; Kinetics; Crk-Associated Substrate Protein; src-Family Kinases; Female; Signal Transduction

Steroid hormones bind to their receptors and trans-activate target genes. Rapid non-genomic action of steroid hormones has been proposed in addition to the one at the genomic level. Estrogen has been described to activate c-Src kinase and this activation has been shown to be responsible for estrogen-dependent mitogenicity. A major substrate of c-Src kinase activity is the cytoskeletal protein p130Cas, originally identified in v-Src-transformed cells. We show that in the human breast carcinoma T47D cells, upon estrogen treatment, p130Cas rapidly and transiently associates with the estrogen receptor α in a multi-molecular complex containing the c-Src kinase and the p85 subunit of PI 3-kinase. Association of p130Cas with the estrogen receptor α occurs within 3 minutes of estrogen treatment and is dependent on c-Src kinase activation. Transient overexpression of p130Cas in T47D cells increases estrogen-dependent Src kinase and Erk1/2 MAPKs activities and accelerates their kinetics of stimulation. A similar effect was detected on estrogen-dependent cyclin D1 expression, suggesting a role for p130Cas in regulating estrogen-dependent cell cycle progression. Double-stranded small RNA interference (siRNA) by silencing endogenous p130Cas protein, was sufficient to inhibit estrogen-dependent Erk1/2 MAPKs activity and cyclin D1 induction, demonstrating the requirement of p130Cas in such events. Therefore, our data show that the adaptor protein p130Cas associates with the estrogen receptor transducing complex, regulating estrogen-dependent activation of c-Src kinase and downstream signaling pathways.