Loss of α10β1 integrin expression leads to moderate dysfunction of growth plate chondrocytes

• DOI: 10.1242/jcs.01678
• Publication Date: 2005-02-16T01:29:03Z
• Authors: Therese Bengtsson; Attila Aszodi; Claudia Nicolae; Ernst B. Hunziker; Evy Lundgren-Åke...; Reinhard Fässler
• Keywords: Integrins; 0303 health sciences; Bone Development; Genotype; Integrin beta1; Green Fluorescent Proteins; Bone and Bones; Mice; 03 medical and health sciences; Chondrocytes; Animals, Newborn; Cell Adhesion; Animals; Immunoprecipitation; Collagen; Growth Plate; Coloring Agents; Integrin alpha Chains; Alleles; Gene Deletion; In Situ Hybridization; Cell Proliferation

Integrin alpha10beta1 is a collagen-binding integrin expressed on chondrocytes. In order to unravel the role of alpha10 during development, we generated mice carrying constitutive deletion gene. The mutant had normal lifespan and were fertile but developed growth retardation long bones. Analysis skeleton revealed defects in plate after birth characterized by disturbed columnar arrangement chondrocytes, abnormal chondrocyte shape reduced proliferation. Electron microscopy plates from newborn an increased number apoptotic chondrocytes density collagen fibrillar network compared these structures control mice. These results demonstrate that plays specific morphogenesis function.