Upregulation of MMP-9 in MDCK epithelial cell line in response to expression of the Snail transcription factor

• DOI: 10.1242/jcs.02465
• Publication Date: 2005-08-03T18:23:54Z
• Authors: Mireia Jordà; David Olmeda; Antònia Vinyals; Eva Valero; Eva Cubillo; Ana Llorens; Amparo Cano; Àngels Fabra
• Keywords: Transcription; Response element

Overexpression of the transcription factor Snail in epithelial MDCK cells promotes epithelial-mesenchymal transition (EMT) and acquisition an invasive phenotype. We report here that expression is associated with increase promoter activity matrix metalloproteinase MMP-9. The effect silencing on MMP-9 corroborates this finding. Induced by driven a mechanism dependent MAPK phosphoinositide 3-kinase (PI3K) signalling pathways. Although other regions were required for complete stimulation Snail, minimal fragment (nucleotides -97 to +114) produces response following increased phosphorylation Sp-1 either or Ets-1 binding GC-box elements contained region. dominant negative form MEK decreased these complexes. A moderate nuclear κB (NFκB) upstream region (nucleotide -562) was also observed Snail-expressing cells. Interestingly, oncogenic H-Ras (RasV12) synergistically co-operates induction expression. Altogether, results indicate activated it might explain, at least part, properties