Ubiquitin C-terminal hydrolase L1 regulates the morphology of neural progenitor cells and modulates their differentiation

• DOI: 10.1242/jcs.02716
• Publication Date: 2005-12-22T00:33:05Z
• Authors: Mikako Sakurai; Koichi Ayukawa; Rieko Setsuie; Kaori Nishikawa; Yoko Hara; Hiroki Ohashi; Mika Nishimoto; Toshiaki Abe; Yoshihisa Kudo; Masayuki Sekiguchi; Yae Sato; Shunsuke Aoki; Mami Noda; Keiji Wada
• Keywords: Gliogenesis; Nestin; Progenitor; Neuroepithelial cell

Ubiquitin C-terminal hydrolase L1 (UCH-L1) is a component of the ubiquitin system, which has fundamental role in regulating various biological activities. However, functional system neurogenesis not known. Here we show that UCH-L1 regulates morphology neural progenitor cells (NPCs) and mediates neurogenesis. was expressed cultured NPCs as well embryonic brain. Its expression pattern ventricular zone (VZ) changed between day (E) 14 E16, corresponds to transition from gliogenesis. At E14, highly zone, where actively occurs; whereas its prominent cortical plate at E16. very weakly detected VZ start In proliferating NPCs, co-expressed with nestin, marker undifferentiated cells. differentiating cells, early neuronal TuJ1. Furthermore, when induced nestin-positive number length cellular processes progenitors decreased, suggesting were differentiating. addition, derived gad (UCH-L1-deficient) mice had longer compared controls. The ability regulate dependent on binding affinity for but activity; this result also confirmed using gad-mouse-derived NPCs. These results suggest spatially enhances brain by cell morphology.