BMP2 induction of actin cytoskeleton reorganization and cell migration requires PI3-kinase and Cdc42 activity

• DOI: 10.1242/jcs.031286
• Publication Date: 2008-11-12T01:34:52Z
• Authors: Cristina Gamell; Nelson Osses; Ramon Bartrons; Thomas Rückle; Montserrat Camps; José Luis Rosa; Francesc Ventura
• Keywords: CDC42

Bone morphogenetic proteins (BMPs) are potent regulators of several cellular events. We report that exposure C2C12 cells to BMP2 leads an increase in cell migration and a rapid rearrangement the actin filaments into cortical protrusions. These effects required independent parallel activation Cdc42 small GTPase alpha-isoform phosphoinositide 3-kinase (PI3Kalpha), because ectopic expression dominant-negative form or distinct pharmacological PI3K inhibitors abrogated these responses. Furthermore, we demonstrate activates different group I II PAK isoforms as well LIMK1 with similar kinetics activation. LIMK1, measured by either kinase activity antibodies raised against phosphorylated residues at their loops, were abolished blocking PI3K-signaling pathways. Together, findings suggest signals emanating from BMP receptors involved specific regulation assembly migration.