β-catenin relieves I-mfa-mediated suppression of LEF-1 in mammalian cells

• DOI: 10.1242/jcs.03257
• Publication Date: 2006-11-08T03:38:53Z
• Authors: Weijun Pan; Yingying Jia; Tao Huang; Jiyong Wang; Donglei Tao; Xiaoqing Gan; Lin Li
• Keywords: Beta-catenin; LRP6; LRP5

We have previously shown that beta-catenin interacts with a transcription suppressor I-mfa and, through this interaction, canonical Wnt signaling could relieve I-mfa-mediated suppression of myogenic regulatory factors (MRFs). In study, we found that, based on the factor T-cell factor/lymphoid enhancing factor-1 (TCF/LEF-1) can also be relieved. Our work showed knocking down endogenous expression mimics treatment by inducing myogenesis and increasing reporter gene activity, target MRFs in P19 cells. More importantly, these small interfering RNA (siRNA)-induced effects blocked dominant-negative mutant LEF-1, confirming involvement TCF/LEF-1 pathway. addition, compete for binding to LEF-1 inhibitory overexpression systems. Furthermore, was able reduce levels associated while beta-catenin. All evidence supports conclusion suppress inhibiting may elevating levels, which turn suppression.