Mitochondrial bioenergetics and structural network organization

• DOI: 10.1242/jcs.03381
• Publication Date: 2007-02-14T02:37:09Z
• Authors: Giovanni Benard; Nadège Bellance; Dominic James; Philippe Parrone; Helder Fernandez; Thierry Letellier; Rodrigue Rossignol
• Keywords: Bioenergetics; Crosstalk; Mitochondrial respiratory chain

Mitochondria form a dynamic network, and it remains unclear how the alternate configurations interact with bioenergetics properties. The metabolic signals that link mitochondrial structure to its functional states have not been fully characterized. In this report, we analyze bidirectional relationships between morphology function in living human cells. First, determined effect of fission on energy production by using small interfering RNA (siRNA) targeting DRP1, which revealed importance membrane fluidity control bioenergetics. Second, followed rotenone, specific inhibitor respiratory chain complex I, causes large structural perturbations, once threshold was reached. Last, changes network configuration cells had treated modulators oxidative phosphorylation, fibroblasts from two patients disease where rate, DeltaPsi generation reactive oxygen species (ROS) were measured. Our data demonstrate relationship organization is bidirectional, provide model for analyzing involved crosstalk.