AIMP2 promotes TNFα-dependent apoptosis via ubiquitin-mediated degradation of TRAF2

• DOI: 10.1242/jcs.049767
• Publication Date: 2009-07-08T02:59:21Z
• Authors: Jin Woo Choi; Dae Gyu Kim; Min Chul Park; Jung Yeon Um; Jung Min Han; Sang Gyu Park; Eung-Chil Choi; Sunghoon Kim
• Keywords: TRAF2; Immunoprecipitation

AIMP2 (aminoacyl-tRNA synthetase interacting multifunctional protein 2; also known as JTV-1) was first identified p38 in a macromolecular complex that consisted of nine different aminoacyl-tRNA synthetases and two other auxiliary factors. plays pivotal roles the regulation cell proliferation death. Although previously shown to augment TNFalpha-induced death, its working mechanism this signal pathway not understood. Here, we investigate functional significance mode action TNFalpha signaling. death compromised AIMP2-deficient or -suppressed cells exogenous supplementation augmented apoptotic sensitivity This activity confirmed by AIMP2-dependent increase IkappaB suppression NFkappaB. We found binding TRAF2, key player signaling pathway. association an E3 ubiquitin ligase, c-IAP1, with causing ubiquitin-dependent degradation TRAF2. These findings suggest can mediate pro-apoptotic via downregulation TRAF2 expression.