Dynein and kinesin regulate stress-granule and P-body dynamics

• DOI: 10.1242/jcs.051383
• Publication Date: 2009-10-14T03:59:58Z
• Authors: Mariela Loschi; Claudia C. Leishman; Neda Berardone; Graciela L. Bocca...
• Keywords: Kinesin; Stress granule; Dynactin; Molecular motor

Stress granules (SGs) and P-bodies (PBs) are related cytoplasmic structures harboring silenced mRNAs. SGs assemble transiently upon cellular stress, whereas PBs constitutive further induced by stress. Both foci highly dynamic, with messenger ribonucleoproteins (mRNPs) proteins rapidly shuttling in out. Here, we show that impairment of retrograde transport knockdown mammalian dynein heavy chain 1 (DHC1) or bicaudal D1 (BicD1) inhibits SG formation PB growth without affecting protein-synthesis blockage. Conversely, anterograde kinesin-1 (KIF5B) kinesin light (KLC1) delayed dissolution. Strikingly, dissolution is not required to restore translation. Simultaneous reverted the effect single knockdowns on both PBs, suggesting a balance between opposing movements driven these molecular motors governs Finally, found regulation dynamics conserved Drosophila.