NEK7 is essential for centriole duplication and centrosomal accumulation of pericentriolar material proteins in interphase cells

• DOI: 10.1242/jcs.078089
• Publication Date: 2011-11-19T02:02:59Z
• Authors: Sunghwan Kim; Sungjae Kim; Kunsoo Rhee
• Keywords: Centriole; Centrosome cycle

The centrosomes in dividing cells follow a series of cyclical events duplication and separation, which are tightly linked to the cell cycle. Serine/threonine-protein kinase NEK7 (NEK7) is centrosomal that required for proper spindle formation during mitosis. In this study, we observed centriole was inhibited NEK7-depleted cells. Ectopic expression centrosome-directed led extra centrioles kinase-activity-dependent manner. We also NEK6-expressing cells, suggesting NEK6 might share biological activities induce duplication. pericentriolar material (PCM) proteins were significantly reduced PCM did not accumulate at centrosomes, even if exited mitosis progressed G2 phase. These results revealed essential accumulation cycle stage-specific Furthermore, HeLa depleted S phase retained higher quantity exhibited less severe mitotic phenotype. On basis these results, propose involved recruitment proteins, necessary both pole formation. Our study activity centrosome progression only M phase, but G1