Differential regulation of actin microfilaments by human MICAL proteins

• DOI: 10.1242/jcs.089367
• Publication Date: 2012-02-14T01:46:44Z
• Authors: Sai Srinivas Pana...; Jennifer L. Rohn; Naava Naslavsky; Steve Caplan
• Keywords: Plexin; Actin remodeling; Growth cone; Myofilament

The Drosophila melanogaster MICAL protein is essential for the neuronal growth cone machinery that functions through plexin- and semaphorin-mediated axonal signaling. also involved in regulating myofilament organization synaptic structures, serves as an actin disassembly factor downstream of plexin-mediated repulsion. In mammalian cells there are three known isoforms, MICAL1, MICAL2 MICAL3, well MICAL-like proteins MICAL-L1 MICAL-L2, but little their function, information comes almost exclusively from neural cells. this study we show non-neural human MICALs required normal organization, all regulate stress fibers. Moreover, provide evidence generation reactive oxygen species by crucial actin-regulatory function. However, although MICAL1 auto-inhibited its C-terminal coiled-coil region, remains constitutively active affects These data suggest differential complementary roles microfilament regulation.