Soluble molecules are key in maintaining the immunomodulatory activity of murine mesenchymal stromal cells

• DOI: 10.1242/jcs.093070
• Publication Date: 2012-01-17T03:52:35Z
• Authors: Hua Liu; Kai Lu; Paul Anthony MacAry; Kok Loon Wong; Alexis Heng; Tong Cao; David Michael Kemeny
• Keywords: Ex vivo; Cell therapy

Mesenchymal stromal cells (MSCs) possess both immuno-privileged and immuno-inhibitory properties that contribute to their therapeutic effects. Ex vivo expansion is required obtain sufficient for therapy, but might also alter immunological properties. To date there has been no systematic study of MSC immunobiology during extended culture. Here, we demonstrate immuno-privilege immunosuppressive MSCs change with increasing passage. We although exhibit powerful effects through secretion transforming growth factor-β (TGF-β) induction interleukin-10, these are diminished by a concomitant increase in immunogenicity. Interferon-γ treatment 3 days induced extendedly cultured express significantly higher levels major histocompatibility complex class I. In vivo, this results induce significant delayed-type hypersensitivity reactions allogeneic recipients. Importantly, alleviated isolation the transplanted using semi-permeable barrier. Under conditions, even as many 14 passages still vivo. Furthermore, anti-inflammatory molecule TGF-β secreted were maintained These data shed light on variable transplantation suggest alternative strategies prolonging effect cell-based therapy.