TPPP acts downstream of RhoA-ROCK-LIMK2 to regulate astral microtubule organization and spindle orientation

• DOI: 10.1242/jcs.096818
• Publication Date: 2012-02-11T04:27:03Z
• Authors: Yi-Wen Heng; Hong-Hwa Lim; Theresia Mina; Prayudi Utomo; Shaoping Zhong; Chwee-Teck Lim; Cheng-Gee Koh
• Keywords: Astral microtubules; Cofilin; Actin remodeling

The actin cytoskeleton in eukaryotic cells undergoes drastic rearrangement during mitosis. changes are most obvious the adherent where stress fibres disassembled and cortical network becomes more prominent with concomitant increase cell rigidity as round up enter While regulatory connections between early mitotic events apparent, mechanisms that govern these links not well understood. Here, we report LIMK1 LIMK2, downstream effectors of RhoA/ROCK, regulate centrosome integrity astral microtubules organization respectively. Surprisingly, cofilin involved RhoA/ROCK regulation microtubule organization. Instead, find LIMK2 acts via TPPP microtubule, while both affect focusing. Both phenotypes tightly coupled to spindle orientation cells. Thus, our results reveal a novel link stages