cis-Golgi resident proteins and O-glycans are abnormally compartmentalized in the RER of colon cancer cells

• DOI: 10.1242/jcs.105.3.819
• Publication Date: 2021-04-25T15:34:46Z
• Authors: Gustavo Egea; Clara Franci; Gemma Gambús; Thecla Lesuffleur; Alain Zweibaum; Francisco X. Real
• Keywords: Malignant Transformation; Neoplastic transformation

ABSTRACT Neoplastic transformation is commonly associated with altered glycosylation of proteins and lipids. To understand the basis for mucin glycosylation, we have examined distribution RER markers, a cis-Golgi resident protein, GalNAc α-O-Ser/Thr epitope (Tn) in human colon cancer cells normal colon. In cultured mucin-producing cells, Gal-NAc was found droplets cisternae. addition, Golgi apparatus disorganized proportion 130 kDa protein also abnormally redistributed to RER. The MUC2 intestinal apomucin, disulphide isomerase, α-O-Ser/Thr, analysed tissues. colon, apomucin isomerase were located RER, whereas detected only compartment. contrast, two markers colocalized cells. On these results, propose that redistribution molecules normally present takes place; this alteration may contribute abnormal lipids neoplastic transformation.