Phagocytosis of apoptotic bodies by liver endothelial cells

• DOI: 10.1242/jcs.108.3.967
• Publication Date: 2021-04-25T16:18:35Z
• Authors: Luciana Dini; Alessandro Lentini; Guzman Diez Diez; Marian Rocha; Laura Falasca; Lucia Serafino; Fernando Vidal-Va...
• Keywords: Male; 0303 health sciences; Apoptosis; Receptors, Cell Surface; Rats; Mice; Microscopy, Electron; 03 medical and health sciences; Liver; Phagocytosis; Cell Adhesion; Animals; Carbohydrate Metabolism; Endothelium; Rats, Wistar; Interleukin-1

ABSTRACT Using electron microscopy and cytofluorimetry we studied the role of carbohydrate-specific recognition systems in the interaction of apoptotic bodies with normal and interleukin 1-activated sinusoidal endothelial cells. Microfluorimetric observation of liver tissue sections revealed octadecyl-rhodamine B-labelled apoptotic body binding to the sinu-soidal wall of mouse liver, when they were injected intra-portally. Plate-scanning cytofluorimetry demonstrated that about 20-25% of Acridine Orange-labelled apoptotic bodies could adhere specifically to cultured endothelial cells after 15 minutes of incubation. Adhesion increased to 30% when the cells were incubated for 60 minutes. Using a mixture of galactose/N-acetylglucosamine/mannose as competition solution apoptotic body adhesion was signifi-cantly reduced especially after longer times of incubation, when the percentage of inhibition reached 50%. Following 4 hours exposure of liver endothelial cells to 1 ng/ml human recombinant interleukin-1β adhesion markedly increased after 60 minutes of incubation, whereas the co-incubation of interleukin-1β with the inhibitors brings down the adhesion to basal values obtained in controls. Electron microscopic observation of the adhesion process showed that the number of endothelial cells binding apoptotic bodies gradually increased from low to high values with time. After 60 minutes of incubation, the majority of apoptotic bodies were seen inside phagosomes and only a few remained at the cell surface. Liver endothelial cells bound and endocytosed apoptotic bodies through carbohy-drate-specific receptors. Moreover, this scavenger action was interleukin-1 enhanced, thus suggesting its possible activation during inflammatory and immune processes.