Fibronectin provides a conduit for fibroblast transmigration from collagenous stroma into fibrin clot provisional matrix

• DOI: 10.1242/jcs.110.7.861
• Publication Date: 2021-04-25T16:51:06Z
• Authors: Doris Greiling; Richard A. F. Clark
• Keywords: Granulation tissue

ABSTRACT After injury, the wound space is filled with a fibrin/fibronectin clot containing growth factors released by platelets and monocytes. In response to these factors, fibroblasts migrate into fibrin contribute formation of granulation tissue. The functional mechanisms allowing leave collagenous matrix normal connective tissue invade provisional have not been fully defined. To investigate we established new in vitro model which simulates specific aspects early healing, that is, migration from threedimensional collagen clot. This transmigration could be induced physiological concentrations platelet releasate or platelet-derived factor BB (PDGF-BB) concentration-dependent manner. At 24 hours irradiated invaded gel almost as well non-irradiated cells, indicating was independent proliferation. Plasminogen its activators appear necessary for invasion since protease inhibitors decreased amount migration. These serine proteases, however, were exit migrated out onto surface coated fibrils even presence inhibitors. Removal fibronectin (FN) either markedly number migrating suggesting FN provides conduit transmigration. Cell movement inhibited RGD peptide, monoclonal antibodies against subunits α5β1 αvβ3 integrin receptor. Thus, requirements fibroblast collagen-rich fibrin-rich matrices, such occurs partially defined using an paradigm this important biologic process.