Septins 2, 7, and 9 and MAP4 co-localize along the axoneme in the primary cilium and control ciliary length

• DOI: 10.1242/jcs.111377
• Publication Date: 2013-04-10T02:19:09Z
• Authors: Rania Ghossoub; Qicong Hu; Marion Failler; Marie-Christine R...; Benjamin Spitzbarth; Serge Mostowy; Uwe Wolfrum; Sophie Saunier; Pascale Cossart; W. James Nelson; Alexandre Benmerah
• Keywords: Septin; Axoneme; Ciliogenesis

Septins are a large, evolutionarily conserved family of GTPases that form hetero-oligomers and interact with the actin-based cytoskeleton microtubules. They involved in scaffolding functions, diffusion barriers budding yeast, sperm flagellum base primary cilia kidney epithelial cells. We investigated role septins cilium retinal pigmented (RPE) cells, found SEPT2 forms 1∶1∶1 complex SEPT7 SEPT9 three members this co-localize along length axoneme. Similar to observations depletion cilium-localized by siRNA-based approaches inhibited ciliogenesis. MAP4, which is binding partner controls accessibility microtubules, was also localized axoneme where it appeared negatively regulate ciliary length. Taken together, our data provide new insights into functions regulation MAP4 organization microtubule-based activities