Examining the relationship between the gelatinolytic balance and the invasive capacity of endothelial cells

• DOI: 10.1242/jcs.112.9.1283
• Publication Date: 2021-04-25T17:29:46Z
• Authors: Alain Puyraimond; Jonathan B. Weitzman; Emeline Babiole; Suzanne Menashi
• Keywords: 0301 basic medicine; 0303 health sciences; Neovascularization, Pathologic; Transcription, Genetic; Reverse Transcriptase Polymerase Chain Reaction; Metalloendopeptidases; Tissue Inhibitor of Metalloproteinases; 03 medical and health sciences; Cell Movement; Gelatinases; Transforming Growth Factor beta; Animals; Tetradecanoylphorbol Acetate; Cattle; Endothelium, Vascular

ABSTRACT Angiogenesis and the formation of new blood vessels requires coordinated regulation of matrix proteolysis and endothelial cell migration. Cellular proteolytic capacity is the balance between secreted matrix metalloproteinases (MMP) and their inhibitors (TIMPs). We have examined the regulation of the gelatinase/TIMP balance by transforming growth factor-β1 (TGF-β1) and phorbol myristate acetate (PMA) in bovine endothelial cells. The low constitutive expression of gelatinase A/MMP-2 was upregulated by TGF-β1 in a dose-dependent manner. Gelatinase B/MMP-9 was only detected upon treatment with either PMA or TGF-β1. However, addition of both factors together revealed a striking synergistic effect causing upregulation of MMP-9 and downregulation of TIMPs, thereby increasing the net MMP-9/TIMP balance and the gelatinolytic capacity. These effects were observed at both the protein and mRNA levels. We demonstrate that changes in different members of the Jun oncogene family with distinct transactivation properties may account for this synergistic effect. We investigated the contribution of these changes in gelatinolytic balance to endothelial cell migration and invasion. The endothelial cells showed increased cell motility in response to PMA, but the addition of TGF-β1 had an inhibitory effect. Hence, regulation of the MMP-9/TIMP balance failed to correlate with the migratory or invasive capacity. These results question a direct role for MMP-9 in endothelial cell motility and suggest that gelatinases may contribute in alternative ways to the angiogenic process.