Fast transport of neurofilament protein along microtubules in squid axoplasm

Intermediate filament 0303 health sciences Time Factors Molecular Sequence Data Decapodiformes Fluorescent Antibody Technique Kinesins Microtubule Neurofilament Kinesin Axonal Transport Microtubules Axons Epitopes Kinetics Protein Subunits Protein Transport 03 medical and health sciences Neurofilament Proteins Animals Amino Acid Sequence Biology Cytoskeleton Axonal transport
DOI: 10.1242/jcs.113.22.3939 Publication Date: 2021-04-25T18:28:18Z
ABSTRACT
ABSTRACT Using squid axoplasm as a model system, we have visualized the fast transport of non-filamentous neurofilament protein particles along axonal microtubules. This transport occurs at speeds of 0.5-1.0 μm/second and the majority of neurofilament particles stain with kinesin antibody. These observations demonstrate, for the first time, that fast (0.5-1.0 μm/second) transport of neurofilament proteins occurs along microtubules. In addition, our studies suggest that neurofilament protein can be transported as non-membrane bound, nonfilamentous subunits along axons, and that the transport is kinesin-dependent. Microtubule-based fast transport might therefore provide a mechanism for the distribution and turnover of neurofilament, and perhaps other cytoskeletal proteins, throughout neurons.
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