Fast transport of neurofilament protein along microtubules in squid axoplasm
Intermediate filament
0303 health sciences
Time Factors
Molecular Sequence Data
Decapodiformes
Fluorescent Antibody Technique
Kinesins
Microtubule
Neurofilament
Kinesin
Axonal Transport
Microtubules
Axons
Epitopes
Kinetics
Protein Subunits
Protein Transport
03 medical and health sciences
Neurofilament Proteins
Animals
Amino Acid Sequence
Biology
Cytoskeleton
Axonal transport
DOI:
10.1242/jcs.113.22.3939
Publication Date:
2021-04-25T18:28:18Z
AUTHORS (5)
ABSTRACT
ABSTRACT
Using squid axoplasm as a model system, we have visualized the fast transport of non-filamentous neurofilament protein particles along axonal microtubules. This transport occurs at speeds of 0.5-1.0 μm/second and the majority of neurofilament particles stain with kinesin antibody. These observations demonstrate, for the first time, that fast (0.5-1.0 μm/second) transport of neurofilament proteins occurs along microtubules. In addition, our studies suggest that neurofilament protein can be transported as non-membrane bound, nonfilamentous subunits along axons, and that the transport is kinesin-dependent. Microtubule-based fast transport might therefore provide a mechanism for the distribution and turnover of neurofilament, and perhaps other cytoskeletal proteins, throughout neurons.
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