Calcium puffs are generic InsP3-activated elementary calcium signals and are downregulated by prolonged hormonal stimulation to inhibit cellular calcium responses

• DOI: 10.1242/jcs.114.22.3979
• Publication Date: 2021-04-26T00:26:20Z
• Authors: Stephen C. Tovey; Patrick de Smet; Peter Lipp; David Thomas; Kenneth W. Young; Ludwig Missiaen; Humbert De Smedt; Jan B. Parys; Michael J. Berridge; Jan Thuring; Andrew Holmes; Martin D. Bootman
• Keywords: Calcium Signaling; Inositol trisphosphate; Inositol phosphate; Cell type; Inositol trisphosphate receptor

Elementary Ca2+ signals, such as ‘Ca2+ puffs’, which arise from the activation of inositol 1,4,5-trisphosphate receptors, are building blocks for local and global signalling. We characterized puffs in six cell types that expressed differing ratios three receptor isoforms. The amplitudes, spatial spreads kinetics events were similar each types. resemblance these suggests they a generic elementary signal and, furthermore, different isoforms functionally redundant at level subcellular Hormonal stimulation SH-SY5Y neuroblastoma cells HeLa several hours downregulated expression concomitantly altered properties puffs. amplitude duration substantially reduced. In addition, number puff sites active during onset wave declined. consequence changes was displayed lower propensity to trigger regenerative waves. Therefore, underlie signalling diverse focal points regulation cellular responses.