Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility

• DOI: 10.1242/jcs.161679
• Publication Date: 2015-01-15T05:51:59Z
• Authors: Tingting Song; Yi Zheng; Yarong Wang; Zachary Katz; Xin Liu; Shaoying Chen; Robert H. Singer; Wei Gu
• Keywords: Molecular motor; Messenger RNP; Cell polarity; P-bodies; Kinesin

ZBP1-modulated localization of β-actin mRNA enables a cell to establish polarity and structural asymmetry. Although the mechanism has been well established, underlying how specific molecular motor contributes transport ZBP1 (also known as IGF2BP1) complex in non-neuronal cells remains elusive. In this study, we report isolation identification KIF11, microtubule motor, which physically interacts with is component messenger ribonucleoprotein particles (mRNPs). We show that KIF11 colocalizes mRNA, ability dependent on ZBP1. characterize corresponding regions mediate interaction two proteins vitro vivo. Disruption vivo delocalizes affects migration. Our study reveals by particular mediates an mRNP through direct mRNA-binding protein.