Role of Securin, Separase and Cohesins in female meiosis and polar body formation in Drosophila

• DOI: 10.1242/jcs.179358
• Publication Date: 2015-12-17T03:09:54Z
• Authors: Zhihao Guo; Osamah Batiha; Mohammed Bourouh; Eric Fifield; Andrew Swan
• Keywords: Separase; Securin

Chromosome segregation in meiosis is controlled by a conserved pathway that culminates Separase-mediated cleavage of the α-kleisin Rec8, leading to dissolution cohesin rings. Drosophila has no gene encoding and absence known Separase target raises question whether its regulator Securin (Pim Drosophila) are important meiosis. Here, we investigate role Securin, complex female using fluorescence situ hybridization against centromeric arm-specific sequences monitor cohesion. We show destruction activity required for timely release arm cohesion anaphase I centromere-proximal II. They also on polar body chromosomes. Cohesion chromosomes depends components SMC3 mitotic Rad21 (also called Vtd Drosophila). provide cytological evidence meiosis, whereas Rad21, agreement with recent findings, not. conclude regulated Securin-Separase targets diverged target, possibly within complex.