Complexes of plexin-A4 and plexin-D1 convey semaphorin-3C signals to induce cytoskeletal collapse in the absence of neuropilins

• DOI: 10.1242/jcs.208298
• Publication Date: 2018-04-16T10:25:14Z
• Authors: Tatyana Smolkin; Inbal Nir-Zvi; Nerri Duvshani; Yelena Mumblat; Ofra Kessler; Gera Neufeld
• Keywords: Plexin; SEMA3A; Neuropilin

Class-3 semaphorin guidance factors bind to receptor complexes containing neuropilin and plexin receptors. A may several somewhat different constituents, resulting in diverse effects on cell migration. U87MG glioblastoma cells express both neuropilins the four class-A plexins. Here, we show that these respond Sema3A or Sema3B by cytoskeletal collapse contraction but fail contract response Sema3C, Sema3D, Sema3G Sema3E, even when plexins are overexpressed cells. In contrast, expression of recombinant plexin-D1 enabled semaphorins. Surprisingly, unlike Sema3D Sema3G, Sema3C also induced repulsion plexin-D1-expressing which were knocked out using CRISPR/Cas9. absence neuropilins, EC50 was 5.5 times higher, indicating function as enhancers plexin-D1-mediated signaling not absolutely required for signal transduction. Interestingly, plexin-A4 formed with plexin-D1, addition enable Sema3C-induced