Abscisic acid – an anti-angiogenic phytohormone that modulates the phenotypical plasticity of endothelial cells and macrophages

• DOI: 10.1242/jcs.210492
• Publication Date: 2018-02-08T09:44:26Z
• Authors: Julienne Chaqour; Sangmi Lee; Aashreya Ravichandra; Brahim Chaqour
• Keywords: 0301 basic medicine; Fibrin; Macrophages; Cell Plasticity; Endothelial Cells; Neovascularization, Physiologic; Cell Differentiation; Macrophage Activation; Retinal Neovascularization; Models, Biological; Retina; 3. Good health; Mice; 03 medical and health sciences; Fetus; Phenotype; Animals, Newborn; Plant Growth Regulators; Cell Movement; Animals; Gels; Abscisic Acid; Cell Proliferation

ABSTRACT Abscisic acid (ABA) has shown anti-inflammatory and immunoregulatory properties in preclinical models of diabetes inflammation. Herein, we studied the effects ABA on angiogenesis, a strictly controlled process that, when dysregulated, leads to severe angiogenic disorders including vascular overgrowth, exudation, cellular inflammation organ dysfunction. By using 3D sprouting assay, show that effectively inhibits migration, growth expansion endothelial tubes without affecting cell viability. Analyses retinal vasculature developing normoxic hyperoxic mice challenged by oxygen toxicity reveal exogenously administered stunts development regeneration blood vessels. In these models, downregulates (EC)-specific migratory genes, interferes with tip stalk specification, hinders function filopodial protrusions required for precise guidance sprouts. addition, skews macrophage polarization towards M1 phenotype characterized anti-angiogenic marker expression. accordance this, treatment accelerates macrophage-induced programmed regression fetal These findings protective functions against neovascular through modulation EC plasticity, suggesting potential utility as vasoproliferative diseases.