The molecular chaperone Hsp90 regulates heterochromatin assembly through stabilizing multiple complexes in fission yeast

• DOI: 10.1242/jcs.244863
• Publication Date: 2020-06-04T15:49:32Z
• Authors: Li Sun; Xiao-Min Liu; Wen-Zhu Li; Yuan-Yuan Yi; Xiangwei He; Yamei Wang; Quan-Wen Jin
• Keywords: Heterochromatin protein 1; Schizosaccharomyces; Argonaute

In the fission yeast Schizosaccharomyces pombe, both RNAi machinery and RNAi-independent factors mediate transcriptional posttranscriptional silencing heterochromatin formation. Here, we show that of reporter genes at major native heterochromatic loci (centromeres, telomeres, mating-type locus rDNA regions) an artificially induced is alleviated in a hsp90 mutant, hsp90-G84C Also, H3K9me2 enrichment regions, especially subtelomeres, compromised, suggesting assembly defects. We further discovered Hsp90 required for stabilization or RNA-induced (RITS) Argonaute siRNA chaperone (ARC) effector complexes, factor Fft3, shelterin complex subunit Poz1 Snf2/HDAC-containing repressor (SHREC). Our ChIP data suggest regulates efficient recruitment methyltransferase/ubiquitin ligase CLRC by to chromosome ends targeting SHREC Fft3 mating type and/or region. Finally, our genetic analyses demonstrated increased spreading restores subtelomeres mutant. Thus, this work uncovers conserved critical promoting RNAi-dependent -independent gene through stabilizing multiple effectors complexes.