Cik1 and Vik1 accessory proteins confer distinct functions to the kinesin-14 Kar3

• DOI: 10.1242/jcs.260621
• Publication Date: 2023-05-19T08:32:41Z
• Authors: Zane J. Bergman; Jonathan J. Wong; David G. Drubin; Georjana Barnes
• Keywords: Kinesin; Spindle pole body

The budding yeast Saccharomyces cerevisiae has a closed mitosis in which the mitotic spindle and cytoplasmic microtubules (MTs), both of generate forces order to faithfully segregate chromosomes, remain separated by nuclear envelope throughout cell cycle. Kar3, kinesin-14, distinct functions on MTs each compartment. Here, we show that two proteins, Cik1 Vik1, form heterodimers with regulate its localization function within along cycle-dependent manner. Using MT dynamics reconstitution assay lysates from cycle-synchronized cells, found Kar3Vik1 induces catastrophes S phase metaphase limits polymerization G1 anaphase. In contrast, Kar3Cik1 promotes pauses G1, while increasing Adapting this track motor protein motility, observed is necessary for Kar3's ability plus-ends metaphase, but, surprisingly, not during These experiments demonstrate how binding partners Kar3 modulate diverse spatially temporally.