Sporidesmin and gliotoxin induce cell detachment and perturb microfilament structure in cultured liver cells

• DOI: 10.1242/jcs.85.1.33
• Publication Date: 2021-04-26T17:13:58Z
• Authors: T. W. Jordan; J. S. Pedersen
• Keywords: Gliotoxin; Pseudopodia

ABSTRACT Changes in cell morphology and adhesion occurred when cultured cells from the rat liver strain C3 were exposed to fungal toxins, sporidesmin or gliotoxin. Both toxins caused loss of attachment plastic tissue culture plates this effect was preceded by actin cables. Other changes included cytoplasmic vacuolation blocked entry into S-phase cycle. Under these conditions [3H]thymidine incorporation also diminished but not detected amount cellular actin, accessibility surface proteins iodination carried out Bolton Hunter method. The observations suggest that disruption microfilaments is one earliest effects on eukaryotic cells.