Glycolysis plays an important role in energy transfer from the base to the distal end of the flagellum in mouse sperm

• DOI: 10.1242/jeb.090985
• Publication Date: 2014-02-28T03:26:46Z
• Authors: Gen L. Takei; Daisuke Miyashiro; Chinatsu Mukai; Makoto Okuno
• Keywords: Male; Mice; 0303 health sciences; 03 medical and health sciences; Adenosine Triphosphate; Energy Transfer; Cell Movement; Sperm Tail; Sperm Motility; Animals; Glycolysis

Many studies have been conducted to elucidate the relationship between energy metabolic pathways (glycolysis and respiration) flagellar motility in mammalian sperm, but contribution of glycolysis sperm has not yet fully elucidated. In present study, we performed detailed analysis mouse for further understanding motility. Mouse maintained vigorous presence substrates either or respiration. By contrast, inhibition by alpha-chlorohydrine caused a significant decrease bend angle bending wave, sliding velocity outer doublet microtubules ATP content even respiratory (pyruvate β-hydroxybutyrate). The are prominent distal part flagellum, indicating that concentration threrein. These results suggest potentially acts as spatial buffering system, transferring (ATP) synthesized respiration at mitochondria located basal flagellum part. order validate glycolytic enzymes can transfer high phosphoryls, calculated intraflagellar profiles adenine nucleotides along computer simulation analysis. result demonstrated involvement maintaining tip flagellum. It is likely plays key role homeostasis only through production also transfer.