Molecular and sensory mechanisms to mitigate sunlight-induced DNA damage in treefrog tadpoles

• DOI: 10.1242/jeb.126672
• Publication Date: 2015-10-08T00:58:10Z
• Authors: André P. Schuch; Victor M. Lipinski; Mauricio B. Santos; Caroline P. Santos; Sinara S. Jardim; Sonia Z. Cechin; Elgion L. S. Loreto
• Keywords: Pyrimidine dimer; Photoprotection; Photolyase; Tadpole (physics)

ABSTRACT The increased incidence of solar ultraviolet B (UVB) radiation has been proposed as an environmental stressor, which may help to explain the enigmatic decline amphibian populations worldwide. Despite growing knowledge regarding UV-induced biological effects in several models, little is known about efficacy DNA repair pathways. In addition, attention given interplay between these molecular mechanisms with other physiological strategies that avoid damage induced by sunlight. Here, lesions doses UVB and UVA were detected genomic samples treefrog tadpoles (Hypsiboas pulchellus) their activity was evaluated. These data complemented monitoring induction apoptosis blood cells tadpole survival. Furthermore, tadpoles’ ability perceive escape from UV wavelengths evaluated additional strategy photoprotection. results show are very sensitive light, could be explained slow rates for both cyclobutane pyrimidine dimers (CPDs) (6,4) pyrimidone photoproducts (6,4PPs). However, they resistant UVA, probably a result activation photolyases during irradiation. Surprisingly, sensory mechanism triggers light avoids generation helps maintain integrity. This work demonstrates genotoxic impact on emphasizes importance minimize caused