The utility and determination of Pcrit in fishes

• DOI: 10.1242/jeb.203646
• Publication Date: 2019-11-13T17:45:13Z
• Authors: Gordon R. Ultsch; Matthew D. Regan
• Keywords: Oxygen; 0106 biological sciences; Oxygen Consumption; Fishes; Animals; Basal Metabolism; 14. Life underwater; Hypoxia; 01 natural sciences; 3. Good health

ABSTRACT The critical O2 tension (Pcrit) is the lowest PO2 at which an animal can maintain some benchmark rate of O2 uptake (ṀO2). This PO2 has long served as a comparator of hypoxia tolerance in fishes and aquatic invertebrates, but its usefulness in this role, particularly when applied to fishes, has recently been questioned. We believe that Pcrit remains a useful comparator of hypoxia tolerance provided it is determined using the proper methods and hypoxia tolerance is clearly defined. Here, we review the available methods for each of the three steps of Pcrit determination: (1) measuring the most appropriate benchmark ṀO2 state for Pcrit determination (ṀO2,std, the ṀO2 required to support standard metabolic rate); (2) reducing water PO2; and (3) calculating Pcrit from the ṀO2 versus PO2 curve. We make suggestions on best practices for each step and for how to report Pcrit results to maximize their comparative value. We also discuss the concept of hypoxia tolerance and how Pcrit relates to a fish's overall hypoxia tolerance. When appropriate methods are used, Pcrit provides useful comparative physiological and ecological information about the aerobic contributions to a fish's hypoxic survival. When paired with other hypoxia-related physiological measurements (e.g. lactate accumulation, calorimetry-based measurements of metabolic depression, loss-of-equilibrium experiments), Pcrit contributes to a comprehensive understanding of how a fish combines aerobic metabolism, anaerobic metabolism and metabolic depression in an overall strategy for hypoxia tolerance.