New Pyrano-Pyrone from Goniothalamus tamirensis Enhances the Proliferation and Differentiation of Osteoblastic MC3T3-E1 Cells

• DOI: 10.1248/cpb.58.521
• Publication Date: 2010-04-12T06:04:33Z
• Authors: Bui Huu Tai; Vu Thi Huyen; Tran Thu Huong; Nguyen Xuan Nhiem; Eun-Mi Choi; Jeong Ah Kim; Pham Quoc Long; Nguyen Manh Cuong; Young Ho Kim
• Keywords: Osteoblasts; Molecular Conformation; Cell Differentiation; Alkaline Phosphatase; 01 natural sciences; Goniothalamus; 0104 chemical sciences; Pyrones; Animals; Collagen; Cell Proliferation

The new pyrano-pyrone, (+)-8-epi-9-deoxygoniopypyrone (1) and (+)-9-deoxygoniopypyrone (2) were isolated from a chloroform extract of Goniothalamus tamirensis leaves. Their absolute stereostructures were discussed and confirmed by using infrared (IR), Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS), one (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR) spectra, Mosher's method, and comparison with the known compounds leiocapin A (3), deoxygoniopypyrone A (4), and (-)-8-epi-9-deoxygoniopypyrone (5). At concentrations of 2.67 microM, compounds 1 and 2 significantly increased the growth of osteoblastic MC3T3-E1 cells and caused a significant elevation of collagen content, alkaline phosphatase activity, and nodule mineralization in the cells (p<0.05). Our data suggest that the enhancement of osteoblast function by 1 and 2 may result in the prevention of osteoporosis.